Journal: Structural Heart

Article Title: Anterior Tilted Deployment of EVOQUE Transcatheter Tricuspid Valve Replacement System: Overcoming Inadequate Implant Depth

doi: 10.1016/j.shj.2026.100816

Figure Lengend Snippet: Left panel top image: 3D intracardiac image and CT simulation demonstrating inadequate right atrial height and a deep capsule position. CT predicted adequate right atrial height of >60 mm. However, the large EV prevented adequate right atrial height and the capsule was too deep. Multiple maneuvers to gain height were insufficient from the right femoral approach, and we converted to a left femoral venous approach. Despite the left femoral approach, the capsule was still deep in the RV at 3.3 cm from the TV annulus and 2.3 cm from the systolic coaptation plane. Left panel bottom image: CT simulation demonstrating the position of the capsule in relation to the EV (∗) and the TV systolic coaptation. Right image: Capsule deep in the RV. The white interrupted line represents the approximate plane of the TV annulus. Abbreviations: A, anterior leaflet; CT, computed tomography; EV, Eustachian valve; P, posterior leaflet; RV, right ventricle; TV, tricuspid valve.

Article Snippet: After informed consent, the patient's TTVR was attempted from a right femoral approach, and a 3D Verisight intracardiac echo (ICE) (Philips, Netherlands) probe was advanced from the left femoral vein using a 12 Fr sheath due to challenging transesophageal echocardiography imaging from the mechanical aortic valve replacement.

Techniques: Computed Tomography

Journal: Structural Heart

Article Title: Anterior Tilted Deployment of EVOQUE Transcatheter Tricuspid Valve Replacement System: Overcoming Inadequate Implant Depth

doi: 10.1016/j.shj.2026.100816

Figure Lengend Snippet: Left image: ICE showing the PA trajectory with the posterior aspect of the capsule edge just beneath the systolic coaptation plane. Right image: Capsule gap created with a pronounced PA trajectory to gain RA height. The white interrupted line represents the approximate plane of the TV annulus. Abbreviations: ICE, intracardiac echo; PA, posterior-to-anterior; RA, right atrium; TV, tricuspid valve.

Article Snippet: After informed consent, the patient's TTVR was attempted from a right femoral approach, and a 3D Verisight intracardiac echo (ICE) (Philips, Netherlands) probe was advanced from the left femoral vein using a 12 Fr sheath due to challenging transesophageal echocardiography imaging from the mechanical aortic valve replacement.

Techniques:

Journal: Structural Heart

Article Title: Anterior Tilted Deployment of EVOQUE Transcatheter Tricuspid Valve Replacement System: Overcoming Inadequate Implant Depth

doi: 10.1016/j.shj.2026.100816

Figure Lengend Snippet: Left image: ICE multiplanar reconstruction images. The anchors are peeking out of the capsule with a pronounced anterior trajectory and the tip of the capsule at the anterior septal commissure in the RVOT. Right image: The entire system is retracted posteriorly to avoid chordal entanglement while maintaining the anterior trajectory. Abbreviations: A, anterior; ICE, intracardiac echo; L, lateral; P, posterior; RVOT, right ventricle outflow tract; S, septal.

Article Snippet: After informed consent, the patient's TTVR was attempted from a right femoral approach, and a 3D Verisight intracardiac echo (ICE) (Philips, Netherlands) probe was advanced from the left femoral vein using a 12 Fr sheath due to challenging transesophageal echocardiography imaging from the mechanical aortic valve replacement.

Techniques:

Journal: Structural Heart

Article Title: Anterior Tilted Deployment of EVOQUE Transcatheter Tricuspid Valve Replacement System: Overcoming Inadequate Implant Depth

doi: 10.1016/j.shj.2026.100816

Figure Lengend Snippet: Top panel: Progressive exposure of the anchors and ventricular flip of the anchors while simultaneously adding primary flex to correct the anterior trajectory and become coaxial to the TV annular plane. The white interrupted line represents the approximate plane of the TV annulus. Bottom panel: ICE multiplanar reconstruction images demonstrating the progressive ventricular flip of the EVOQUE anchors. Abbreviation: ICE, intracardiac echo; TV, tricuspid valve.

Article Snippet: After informed consent, the patient's TTVR was attempted from a right femoral approach, and a 3D Verisight intracardiac echo (ICE) (Philips, Netherlands) probe was advanced from the left femoral vein using a 12 Fr sheath due to challenging transesophageal echocardiography imaging from the mechanical aortic valve replacement.

Techniques:

Journal: Structural Heart

Article Title: Anterior Tilted Deployment of EVOQUE Transcatheter Tricuspid Valve Replacement System: Overcoming Inadequate Implant Depth

doi: 10.1016/j.shj.2026.100816

Figure Lengend Snippet: Left image: ICE image of the valve deployed. Right image: Fluoroscopy of the valve deployed. The white interrupted line represents the approximate plane of the TV annulus. Abbreviation: ICE, intracardiac echo; TV, tricuspid valve.

Article Snippet: After informed consent, the patient's TTVR was attempted from a right femoral approach, and a 3D Verisight intracardiac echo (ICE) (Philips, Netherlands) probe was advanced from the left femoral vein using a 12 Fr sheath due to challenging transesophageal echocardiography imaging from the mechanical aortic valve replacement.

Techniques:

Journal: Molecular Therapy. Nucleic Acids

Article Title: Dual inhibition of intercellular adhesion molecule-1 and nucleolin reduces RSV infection efficiency in human respiratory organoids

doi: 10.1016/j.omtn.2026.102932

Figure Lengend Snippet: ICAM-1- and EGFR-KO iPSCs were established using CRISPR-Cas9 system (A) ICAM-1-knockout (KO) and EGFR-KO iPSCs were generated using the CRISPR-Cas9 system. Bar plots represent the indel distribution for ICAM-1 and EGFR. (B) Phase images of wild-type (WT), ICAM-1-KO, and EGFR-KO iPSCs. Scale bars, 100 μm. (C) Immunofluorescence analysis of OCT3/4 (red) expression in WT, ICAM-1-KO, and EGFR-KO iPSCs. Nuclei were counterstained with DAPI (blue). Scale bars, 100 μm. (D) The expression levels of ICAM-1 and EGFR in WT, ICAM-1-KO, and EGFR-KO iPSC-derived respiratory organoids were measured by RT-qPCR. Data are shown as mean ± SD ( n = 3, technical replicates); two-tailed Student’s t test (∗∗ p < 0.01). (E) The expression levels of ICAM-1, EGFR, and β-actin in WT, ICAM-1-KO, and EGFR-KO iPSC-derived respiratory organoids were measured by the capillary-based immunoassay.

Article Snippet: Analysis was done using inference of CRISPR edits (ICE) ( https://www.synthego.com/help/ice ) showed that the KO scores for ICAM-1-KO and EGFR-KO iPSCs were nearly 100 ( A and A).

Techniques: CRISPR, Knock-Out, Generated, Immunofluorescence, Expressing, Derivative Assay, Quantitative RT-PCR, Two Tailed Test

Journal: Poultry Science

Article Title: High genotoxicity of CRISPR/Cas9 versus limited efficacy of CRISPRi in chicken primordial germ cells

doi: 10.1016/j.psj.2026.106722

Figure Lengend Snippet: CRISPR/Cas9 shows high editing efficiency in PGCs. (A) Phase-contrast images of cultured PGCs isolated from embryonic blood, showing typical colony morphology after 3 weeks in vitro . Left: male PGC colony; right: female PGC colony. Scale bar = 20 µm. (B) Immunofluorescence for germ cell markers in PGCs. These cells (male line shown) strongly express SSEA-1 (green, cell surface) and VASA/DDX4 (red, cytoplasm), even after long-term culture (>50 days). Nuclei are counterstained with DAPI (blue). Scale bar = 5 µm. (C) Representative fluorescence microscopy of EGFP + PGCs 5 days after co-electroporation with Cas9 and sgRNAs targeting EGFP (gEGFP1+2). Left: cells electroporated with Cas9 mRNA at 1 µg, 2 µg, or 3 µg (with constant gRNA amount). Right: cells electroporated with Cas9 protein (RNP complex) at equivalent molar doses (1:1.2 Cas9:sgRNA ratio). In both mRNA and protein conditions, higher Cas9 doses result in loss of EGFP fluorescence and reduced cell numbers (rounding and death) compared to lower doses. Scale bar = 20 µm. (D) Flow cytometry analysis of EGFP fluorescence and cell viability in edited versus control PGCs. Left: histogram overlays of EGFP intensity for control (untreated EGFP + PGCs, gray) vs. CRISPR-edited cells (green). Cas9-edited populations shift toward lower fluorescence, indicating EGFP knockout. Upper right: bar graph quantifying the percentage of EGFP + cells in each group (mean ± SEM, n = 3). Both Cas9 mRNA and Cas9 protein treatments caused a dose-dependent decrease in the fraction of EGFP-expressing cells compared to control (p-values are indicated in the figure by one-way ANOVA). Lower right: plot showing the percentage of live cells recovered during flow cytometry. Higher Cas9 doses correlate with reduced live-cell recovery, reflecting CRISPR-induced cytotoxicity in PGCs. Statistical significance was determined by one-way ANOVA (p-values are indicated in the figure).

Article Snippet: The amplicons were subjected to Sanger sequencing, and sequencing traces were analyzed using the Inference of CRISPR Edits (ICE) software tool (v3.0, Synthego).

Techniques: CRISPR, Cell Culture, Isolation, In Vitro, Immunofluorescence, Fluorescence, Microscopy, Electroporation, Flow Cytometry, Control, Knock-Out, Expressing, Cell Recovery

Journal: Poultry Science

Article Title: High genotoxicity of CRISPR/Cas9 versus limited efficacy of CRISPRi in chicken primordial germ cells

doi: 10.1016/j.psj.2026.106722

Figure Lengend Snippet: CRISPR/Cas9 induces DNA damage and apoptosis in PGCs. (A) Flow cytometry analysis 24 h after electroporation, quantifying the proportion of Annexin V + /PI + cells. The horizontal axis indicates PI and the vertical axis Annexin V. The upper-left quadrant (Annexin V + /PI + ) represents late apoptotic cells, and the lower-right quadrant (Annexin V + only) represents early apoptotic cells. Upper panels: results after electroporation with Cas9 + various gRNAs; lower panels: results with dCas9 + various gRNAs. (B) Bar graph of Annexin V + /PI + percentages across groups. Cas9 editing induced a highly significant increase in late apoptosis. (C) γ-H 2 AX foci (green) detected by immunofluorescence 24 h after electroporation. Foci appear as discrete nuclear puncta; nuclei are counterstained with DAPI (blue). Scale bar = 10 µm. (D) Quantification of γ-H 2 AX foci per cell. Cas9 targeting resulted in a significant increase in γ-H 2 AX foci per cell, whereas dCas9 with sgRNA did not. Statistical significance determined by one-way ANOVA (p-values are indicated in the figure).

Article Snippet: The amplicons were subjected to Sanger sequencing, and sequencing traces were analyzed using the Inference of CRISPR Edits (ICE) software tool (v3.0, Synthego).

Techniques: CRISPR, Flow Cytometry, Electroporation, Immunofluorescence

Journal: Poultry Science

Article Title: High genotoxicity of CRISPR/Cas9 versus limited efficacy of CRISPRi in chicken primordial germ cells

doi: 10.1016/j.psj.2026.106722

Figure Lengend Snippet: CRISPRi has limited efficacy in gene knockdown in PGCs. (A) Schematic of the CRISPR interference (CRISPRi) system. (i) The PGK-CRISPRi-EGFP plasmid expresses dCas9-KRAB (catalytically inactive Cas9 fused to the KRAB repressor) and an EGFP marker under a constitutive PGK promoter. (ii) The gCAG-mCherry plasmid carries a U6.3 promoter–driven sgRNA targeting the CAG promoter and a CAG-driven mCherry reporter. (iii) Co-transfection strategy: dCas9-KRAB (plasmid i) is expressed in the cell, and the sgRNA (plasmid ii) guides it to the CAG promoter in the mCherry cassette, silencing mCherry transcription. (B) Summary of CRISPRi reporter knockdown efficacy in human 293T cells vs. chicken cells. Bars show the percentage of mCherry + cells in each condition (no sgRNA, mock control, +gCAG sgRNA). In 293T cells, introducing the CAG-targeting sgRNA significantly reduces the mCherry + fraction relative to controls, whereas in DF-1 cells the mCherry + percentage remains unchanged, and in PGCs only a slight decrease is observed. (C) Expression of the dCas9-KRAB-EGFP fusion protein in CRISPRi. Western blot confirmed that dCas9-KRAB-EGFP is only expressed in CRISPRi cells, indicating the successful construction of CRISPRi stable PGC cell lines. Blank: Untransfected cells served as the negative control. (D) Gene expression following CRISPRi-mediated knockdown in CRISPRi cells. qRT-PCR showed no significant reduction in expression of the target genes for which CRISPRi sgRNAs were designed. Statistical significance was determined by one-way ANOVA (p-values are indicated in the figure).

Article Snippet: The amplicons were subjected to Sanger sequencing, and sequencing traces were analyzed using the Inference of CRISPR Edits (ICE) software tool (v3.0, Synthego).

Techniques: Knockdown, CRISPR, Plasmid Preparation, Marker, Cotransfection, Control, Expressing, Western Blot, Negative Control, Gene Expression, Quantitative RT-PCR

Journal: HardwareX

Article Title: A low-cost three-wavelength illumination system for camera-based photoplethysmography imaging

doi: 10.1016/j.ohx.2026.e00787

Figure Lengend Snippet: Visual representation of connection of Atmel-ICE Debugger to PCB .

Article Snippet: Select Atmel-ICE as the programmer and ISP as the communication interface, then click Apply ( , step 1.).

Techniques:

Journal: HardwareX

Article Title: A low-cost three-wavelength illumination system for camera-based photoplethysmography imaging

doi: 10.1016/j.ohx.2026.e00787

Figure Lengend Snippet: Visual representation of connection of Atmel-ICE Debugger to PCB .

Article Snippet: Visual representation of connection of Atmel-ICE Debugger to PCB . (3) Connect the Atmel-ICE programmer to the power supply. (4) Connect the power supply to control unit PCB.

Techniques:

Journal: HardwareX

Article Title: A low-cost three-wavelength illumination system for camera-based photoplethysmography imaging

doi: 10.1016/j.ohx.2026.e00787

Figure Lengend Snippet: Visual representation of connection of Atmel-ICE Debugger to PCB .

Article Snippet: Firmware Upload Procedure for the Control Unit: Connect the Atmel-ICE programmer to both the computer and the PCB to be programmed, as shown in and . (1) Open the project in Microchip Studio and click the Device Programming icon.

Techniques: